The DAG content needs to be reduced in AWC and AIA neurons during forgetting. A–C, Transgenic rescue of tir-1(tm3036) mutants by expressing a constitutively active form of the egl-30 gene under a pan-neuronal (H20) (A), AWC (odr-1) (B), or AIA (gcy-28.d) promoter (C). Chemotaxis of naive, adapted, and 4 h recovered animals was analyzed. Asterisks indicate significant differences between after adaptation and after 4 h recovery. A, WT: p = 0.000465; tir-1;Ex[H20p::egl-30(gf)]: p = 0.00166 (n = 4, F(6,36) = 7.81, p = 2.00 × 10−5). B, WT: p = 0.00136; tir-1;Ex[odr-1p::egl-30(gf)]: p = 4.40 × 10−5 (n = 6, F(6,60) = 3.81, p = 0.00280). C, WT: p = 3.15 × 10−8 (n = 4, F(6,36) = 6.40, p = 0.000118). D–G, Transgenic rescue of the goa-1(n1134);tir-1(tm3036) mutant's phenotype by expressing the WT goa-1 gene under its own (goa-1) (D), pan-neuronal (H20) (E), AWC (odr-1) (F), or AIA (gcy-28.d) promoter (G). D, WT: p = 8.07 × 10−31; goa-1;tir-1 (Ex-): p = 4.06 × 10−9 (n = 18, F(6,204) = 22.73, p = 1.87 × 10−20). E, WT: p = 2.79 × 10−6; goa-1;tir-1 (Ex-): p = 0.000961 (n = 8, F(6,84) = 8.60, p = 2.71 × 10−7). F, WT: p = 1.09 × 10−7; tir-1 (tm3036): p = 0.00618; goa-1;tir-1 (Ex-): p = 0.000388 (n = 12, F(6,132) = 11.30, p = 3.67 × 10−10). G, WT: p = 3.51 × 10−18; goa-1;tir-1 (Ex-): p = 8.36 × 10−6 (n = 8, F(6,84) = 17.22, p = 6.99 × 10−13). H, Overexpression of goa-1 in AWC neurons prevents WT animals from forgetting. WT: p = 1.91 × 10−13; WT (Ex-): p = 2.62 × 10−6 (n = 8, F(6,84) = 11.00, p = 5.16 × 10−9). Box plots: center line indicates median. Box range, 25th-75th percentiles. Whiskers represent minimum-maximum values. Dots represent the data points. External dots from whisker represent outliers. **p < 0.01; ***p < 0.001; two-way ANOVA with Bonferroni correction.
