Normal neuronal migration and cortical plate thickness in the embryonic prh cortex, while no change in neuronal cell death at P8. A, WT and prh brains at E18 stained with cortical layer-specific markers SATB2 (II–IV), CTIP2 (V), and TBR1 (VI) show no difference in thickness and neuronal cell density of each cortical layer between WT and prh (scale bars: 1000 μm for lower magnification pictures, 100 μm for higher magnification pictures, Student's t test, n = 3 in each group). B, TUNEL/NeuN double staining at P8 in prh brains (top row, veh-WT; bottom row, veh-prh) shows more TUNEL-positive cells in veh-prh than veh-WT, but no change in apoptotic neuronal cell death (i.e., TUNEL/NeuN double staining) between WT and prh (scale bars: 100 μm, n = 3 in each group). C, TUNEL/GFAP double staining at P8 in veh-prh brains shows no apoptotic astrocytic cell death (scale bars: 50 μm, n = 3).