CSMD3-deficient mice exhibit core autistic-like symptoms. A–G, Three-chamber sociability test. A, representative automated tracking of a single mouse in the three-chamber sociability test (10 min duration in each stage) across the WT, CSMD3^−/−, and CSMD3^+/− mice. B–G, Bar graph represents sociability (B), social novelty (C), social preference index (D), social novelty index (E), as well as chamber entries in sociability test (F), and social novelty test (G): F_(2,30) = 2.64 (B), F_(2,30) = 4.43 (C), F_(2,15) = 2.24 (D), F_(2,15) = 7.03 (E), F_(2,30) = 1.67 (F), and F_(2,30) = 2.28 (G). H, Grooming behaviors. I, Marble-burying test. J, Spontaneous digging behaviors. F_(2,24) = 11.41, p = 0.0002 and p = 0.0019 (H); F_(2,30) = 5.30, p = 0.0226 and p = 0.0163 (I); F_(2,19) = 1.71, p = 0.3230 and p = 0.2261 (J), CSMD3^−/− versus WT and CSMD3^+/− versus WT, respectively. K, NOR test. Representative automated tracking of either a single wild-type or CSMD3^−/− mouse in the NOR test (in the 5 min test phase, Trial 2; left) and bar graph of recognition index (right) are shown. F_(1,16) = 7.71, p = 0.0002 for Trial 2, CSMD3^−/− versus WT. Data are presented as the mean ± SEM: n = 6 mice/group (B–G); n = 9–13 mice/group (H–K). *p < 0.05, **p < 0.01, and ***p < 0.001, two-way ANOVA with Tukey's post hoc test (B, C, F, G); two-way ANOVA with Sidak's post hoc test (K); one-way ANOVA with Tukey's post hoc test (D, E, H–J).