Depleting Hrs in prion-infected mice reduces early endosomal proteins. A–E, Representative Western blottings and quantification of endosomal proteins in brain lysates of uninfected and prion-infected Hrs^f/fSyn1-Cre⁻ and Cre⁺ mice. Relative protein levels were normalized to the average of the uninfected Hrs^f/fSyn1-Cre⁻ levels. F, G, Western blottings and quantification of Rab5 in prion-infected Hrs^f/fSyn1-Cre⁻ and Cre⁺ mice at 84 d (F) and 107 d (G) after inoculation. N = 3–8 mice per group. Data shown as mean ± SEM. Two-way ANOVA with Tukey's multiple comparisons post hoc test. B–E, *p < 0.05, **p < 0.01. Unpaired, two-tailed Student's t test (F, G), *p < 0.05, ***p < 0.001.