VU treatment results in elevated RMPs in sRad interneurons at DIV21. A, Whole-cell voltage-clamp recordings of evoked IPSCs in control (CTL) and VU-treated cultures at DIV21. Stimulation electrode was placed in the sRad. Bold represents average evoked IPSCs. Gray represents individual traces. B, C, Paired-pulse ratios (IPSC2/IPSC1 amplitudes) evoked in sRad (MW, p = 0.65) and sPyr (MW, p = 0.21) in CTL and VU-treated cultures at DIV21. D, E, Coefficient of variation (CV) of the first IPSCs evoked in sRad (MW, p = 0.96) and sPyr (MW, p = 0.80) in CTL and VU-treated cultures at DIV21. B–E, Data are from 7 or 8 cells, 4-6 slices, and 3-5 mice per group. F, sEPSC recording in GFP-labeled interneurons in sRad from control (CTL) and VU-treated cultures from GAD65-GFP mice at DIV21. Asterisks indicate sEPSC. G, H, sEPSC frequency (MW; p = 0.85) and amplitude (MW; p = 0.38) in these interneurons. Data are from 15 or 16 cells, 11 slices, and 10 mice per group. I, Whole-cell current-clamp recordings of APs after current injections in sRad interneurons in CTL and VU-treated cultures at DIV21. J, AP firing rates in sRad interneurons in CTL and VU-treated cultures at DIV21 (two-way ANOVA, current injection: p < 0.001, treatment: p = 0.42). K–M, RMP (MW, p = 0.023), AP threshold (UT, p = 0.11), and relative AP (Rel AP) threshold (MW, p = 0.012) in sRad interneurons in CTL and VU-treated cultures at DIV21. J-M, Data are from 13-16 cells, 11 slices, and 10 mice per group. *p < 0.05.