Density of axonal bouton and postsynaptic labeling is comparable across control and trained animals. A, Within- (connected lines) and across-animal comparisons for densities of digitally detected POm boutons in L1 and L5a from acclimation control (Acc) and trained (SAT1) mice. Bouton density was not significantly different between L1 and L5a within layers or across training. Statistical difference was assessed using two-way repeated-measures Acclimation control ANOVA followed by post hoc Tukey's test for comparisons across layers and across training if ANOVA results were significant, F(1,17) = 2.35; p = 0.14. B, As in A but for PSD95 puncta density. PSD95 puncta density was significantly higher in L5a than L1 for both Acc and SAT1 animals, F(1,17) = 102.5; p = 1.29 × 10−8. Tukey's p(across-layer) = 4.17 × 10−7; Tukey's p(across-group) = 0.83. Bar graphs represent mean + SEM; N = 12 Acc and 7 SAT1 mice.
