Developmental AMPAR transmission is decreased in Ppt1^−/− visual cortex. A, Quantification of sEPSC (−60 mV) and sIPSC (+15 mV) frequency from layer 2/3 visual cortical neurons in WT and Ppt1^−/− mice at P14–P15. n = 6–7 cells, 3–4 animals/group. B, Quantification of sEPSC (−60 mV) and sIPSC (+15 mV) frequency from layer 2/3 visual cortical neurons in WT and Ppt1^−/− mice at P28–P30. t test: ****p < 0.0001. n = 9–11 cells, 3–4 animals/group. C, Representative voltage-clamp recordings of sEPSCs and (D) sIPSCs from layer 2/3 visual cortical neurons at P28–P30. E, Representative immunoblots and quantification of total GluA1 levels in lysates from WT and Ppt1^−/− visual cortices for ages P11–P60. Ppt1^−/− data are normalized to the WT mean value at each age and comparisons were made within age. n = 2–4. F, Representative immunoblots and quantification of total GluA2 levels in lysates from WT and Ppt1^−/− visual cortices for ages P11–P60. Ppt1^−/− data are normalized to the WT mean value at each age and comparisons were made within age. n = 2–4. G, Quantification of the combined average of GluA1 and GluA2 levels in each occipital cortex lysate from WT and Ppt1^−/− visual cortices for all ages examined. n = 19/group. H, Representative immunoblots and quantification of GluA1 levels in synaptosomes from WT and Ppt1^−/− visual cortices for ages P11–P60. Ppt1^−/− data are normalized to the WT mean value at each age and comparisons were made within age. n = 2–4. I, Representative immunoblots and quantification of GluA2 levels in synaptosomes from WT and Ppt1^−/− visual cortices for ages P11–P60. Ppt1^−/− data are normalized to the WT mean value at each age and comparisons were made within age. n = 2–4. J, Quantification of the combined average of GluA1 and GluA2 levels in each occipital cortex synaptosome from WT and Ppt1^−/− visual cortices for all ages examined. n = 17/group. K, Representative immunoblots of APEGS-processed synaptosomes probing for GluA1, GluA2, and β-actin at P42. L, Quantification of the palmitoylated/nonpalmitoylated ratio (normalized to β-actin) of GluA1 and (M) GluA2 in APEGS-processed visual cortical synaptosomes. n = 3 mice/group. Data are mean ± SEM.