Table 1.
Key resources table describing all relevant reagents used
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | B6;129-Ppt1tm1Hof/J | Jax stock #004313 | Gupta, PNAS, 2001; RRID: MGI:004313 | |
| Antibody | Mouse monoclonal anti-GluA1 | Millipore Sigma | Catalog #MAB2263; RRID: AB_11212678 | 1:500 for biotinylation, 1:1000 for all else |
| Antibody | Mouse monoclonal anti-GluA2 | UC Davis/NIH NeuroMab Facility | Catalog #75-002; RRID: AB_2232661 | 1:500 for biotinylation, 1:1000 for all else |
| Antibody | Rabbit polyclonal anti-PPT1 | Gift from Sandra Hofmann | 1:500 | |
| Antibody | Mouse monoclonal anti-β-actin | ThermoFisher Scientific | Catalog #A2228; RRID: AB_476697 | 1:2000 |
| Antibody | Donkey anti-rabbit IgG secondary antibody, HRP | ThermoFisher Scientific | Catalog #SA1-200; RRID: AB_325994 | 1:1000 for biotinylation; 1:5000 or 1:10,000 for all else |
| Antibody | Rabbit polyclonal anti-MAP2 | Millipore Sigma | Catalog #AB5622; RRID: AB_91939 | 1:400 |
| Antibody | Peroxidase AffiniPure goat anti-mouse IgG | Jackson ImmunoResearch | Catalog #115-035-146; RRID: AB_2307392 | 1:1000 for biotinylation; 1:5000 or 1:10,000 for all else |
| Antibody | GFP polyclonal antibody, Alexa Fluor 488 | ThermoFisher Scientific | Catalog #A-21311; RRID: AB_221477 | 1:1000 |
| Antibody | Mouse monoclonal anti-transferrin | ThermoFisher Scientific | Catalog #A-11130; RRID:AB_2534136) | 1:500 |
| Antibody | Gt anti-rabbit 633 | ThermoFisher Scientific | Catalog #A-21070; RRID: AB_2535731 | 1:400 |
| Antibody | Gt anti-mouse 488 | ThermoFisher Scientific | Catalog #A28175; RRID: AB_2536161 | 1:400 |
| Virus | AAV8-hDlx-Gq-DREADD-dTomato-Fishell-4 | Vector Biolabs | Addgene (83897-AAV9) | Packaged in AAV8; Gift from Dr. Kuei Tseng |
| Virus | AAV8-pAAV-mDlx-GFP-Fishell-1 | Vector Biolabs | Addgene (83900-AAV9) | Packaged in AAV8; Gift from Dr. Kuei Tseng |
| Virus | AAV9-pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 | Addgene | Catalog #100834-AAV9 | |
| Commercial assay or kit | Dynabeads M-280 Streptavidin | ThermoFisher Scientific | Catalog #11205D | |
| Recombinant DNA reagent | GCaMP3 | Addgene | Plasmid: 22692 | Dr. Looger |
| Recombinant DNA reagent | CAG-mCherry | |||
| Chemical compound, drug | Tetrodotoxin-citrate (TTX) | Tocris | Catalog #1069 | Treatment: 1 μm |
| Chemical compound, drug | NASPM | Tocris | Catalog #2766 | Treatment: 1 μm or 10 μm where indicated |
| Chemical compound, drug | 5-kDa maleimide PEG (for APEGS) | NOF America Corporation | Catalog #ME-050MA | |
| Chemical compound, drug | (+)-Bicuculline | Tocris | Catalog #0130 | Treatment: 20 μm |
| Chemical compound, drug | Clozapine N-oxide | NIH | Gift from Dr. Kuei Tseng | |
| Software, algorithm | Fiji | |||
| Software, algorithm | MATLAB 2020a | MathWorks | EZcalcium (Cantu et al., 2020) | |
| Software, algorithm | Prism 9.0.1 | GraphPad |
