Relative mRNA expression of PrRP receptors in the LHA and DHA in control and suicidal human subjects. A, Schematic of the right human hypothalamus (Mai et al., 2015). White circles represent the size and location of the areas within the LHA (blue) and DHA (beige), from which tissue samples were taken for RT-PCR. f, Fornix; ot, optic tract; 3v, third ventricle. B, RT-PCR data (ΔCt and fold change) of PrRP receptors. LHA_receptors: F_(1,10) = 27.71, p < 0.001. ^#p < 0.001 versus PRLHR within both groups and LHA_groups: F_(1,10) = 14.70, p = 0.003. *p = 0.048 versus controls within both receptors. DHA_receptors: F_(1,10) = 23.05, p < 0.001. ^#p < 0.001 versus PRLHR within both groups and DHA_groups: F_(1,10) = 12.51, p = 0.05. *p = 0.023 and *p = 0.005 versus controls within PRLHR and NPFF2R, respectively. C, RT-PCR data of GAPDH. There is no significant difference between the control and suicidal samples. Two-way repeated-measures ANOVA: B, n = 6/group; C, n = 5 or 6/group. One control DHA sample was excluded for technical reasons. Statistics for all RT-PCR data were calculated using the ΔCt values. Symbols label the results of the Student-Newman-Keuls multiple comparison tests. Bar graphs represent mean ± SEM. Box (indicating the first and third quartiles) and whisker (extending to the minimum and maximum data points) plots are used for showing ΔCt data.