DHX36 and G4-DNA time course for increased G4-DNA targets. DHX36 occupancy in the mPFC following either retention control (RC) or extinction training (EXT) for 10, 30, or 60 conditioned stimuli (10CS, 30CS, 60CS) or equivalent time for RC (RC10, RC30, RC60) with no significant differences observed for (A) Rims4, (B) Kcnb4, and (C) Rictor. There was also no significant change in G4 occupancy following training of the same groups and genes (D) Rims4, (E) Kcnb4, and (F) Rictor. Comparing G4-DNA in activated versus unsorted neurons revealed significant differences between the groups (G). Rims4 (one-way ANOVA F_(3,22) = 4.193, **p < 0.01; Dunnett's post hoc test; RC Arc+ vs EXT Arc+, **p = 0.0056). H, Kcnb4 (one-way ANOVA F_(3,22) = 2.118, p = 0.1270). I, Rictor (one-way ANOVA F_(3,222) = 4.421, **p < 0.01; Dunnett's post hoc test; RC Arc+ vs EXT Arc+, **p = 0.0060).