Experience-dependent nascent RNA expression is modulated by G4-DNA. A, Timeline of infusions of self-deleting Cre lentivirus, training, and 4TU infusions. Following training, brains from 32 animals were extracted, and RNA was labeled and sequenced, producing a distribution of reads across the transcriptome with the highest amount in the exons. B, Volcano plot of 4TU-labeled transcripts that significantly increased or decreased relative to control when DHX36 shRNA was infused (see Extended Data Fig. 5-1 for more details). C, Database for Annotation, Visualization and Integrated Discovery (DAVID) plot comparing category to the p-value for all of the significantly decreased transcripts (D) and all of the significantly increased transcripts. E, A visual representation indicating the percentage of 4TU reads from the significantly decreased and increased transcripts which occurred across introns and exons (exon–intron), only over exons (exon), only over introns (intron), or only over intragenic regions (intragenic). F, The number of significantly increased 4TU transcripts and their distances in kilobases relative to the closest annotated transcription start site.