Identification of dysregulated genes in human DYT1 MNs. A, Schematic shows the workflow of identification of dysregulated genes from iPSC-derived MNs. B, Representative micrographs of cells at different stages during the generation of human MNs. iPSCs, induced pluripotent stem cells; NPCs, neuronal progenitor cells; and MNs, motor neurons. Scale bar, 100 µm. C, Heat map of RNAseq results of DEGs in DYT1 MNs compared with healthy controls. D, Volcano plot shows the upregulated and downregulated DEGs in DYT1 MNs. Six hundred ninety-nine genes (in red) were upregulated, and 589 genes (in blue) were downregulated. E, Some interesting targets have been identified with disrupted gene expression in DYT1 MNs. FPKM, Fragment Per Kilobase of Million mapped read. **p < 0.01. Student’s t tests.