RANBP17 expression decreased in DYT1 neurons. A, The expression levels of different RANBP genes in WT and DYT1 MNs from transcriptomic study. ns, no significant difference; *p < 0.05; ***p < 0.001. Student’s t tests. B, Western blotting shows the indicated protein levels of whole-cell extracts from healthy and DYT1 iPSC-MNs. RANBP17 and β-actin were sequentially probed on the same blot. C, RT-PCR analysis of RANBP17 gene expression levels in MNs generated from indicated two groups of hiPSC lines and healthy MNs with downregulated RANBP17. ns, not significant; ****p < 0.0001. N, three replicates. Student’s t tests. D, Constructions of lentiviral vectors expressing transport reporters and the validation of their subcellular distribution in HEK cells. HST 33342 was used to stain nuclei. Scale bar, 25 µm.