PIC neurons are activated by aversion, stress, and reward. A–P, Schematic diagram (A,E,I,M), heat maps (B,E,J,N), averaged traces (C,G,K,O), and quantification (D,H,L,P) of fluorescent signal in the PIC of GCaMP6 and eYFP mice in response to 1 s air puff, body restraint, being handled by unfamiliar experimenter, and sweet water (20% sucrose) licking. D, t = 3.08, p = 0.0067. H, t = 3.80, p = 0.0014. L, t = 5.51, p < 0.0001. P, t = 2.25, p = 0.028. n = 5 mice in each group.