WT and TARDBPQ331K^+/− iPSC are differentiated into motor neurons expressing pan-neuronal and motor neuron–specific marker(s). A, Differentiation schematic illustrating the time-course treatment of small-molecule cocktails for promoting motor neuron differentiation from iPSCs. B, Flow cytometry data of Day 21 cultured NPCs from each group. Cells were stained with antibodies against human nerve growth factor receptors CD271 and CD44. C, Immunocytochemistry data of Day 27 cultures from each group, showing the expression of motor neuron–specific (ISL-1) and pan-neuronal (TuJ1) markers.