
Suppression of CD8+ T-cell proliferation by coculturing with IFNγ-treated neurons. A, Schematic of the cells and antibodies used (left panel). CFSE-labeled CD8+ T-cells were stimulated by incubating them in anti-CD3-coated plates for 5 d, in the absence or presence of IFNγ-treated iPSC-derived neurons. Quantification of CFSE-labeled CD8+ T-cell proliferation is depicted in each histogram (right panels). B, The result summarized in the bar graph showing % of proliferating T-cells.




