Human Motor Neurons Elicit Pathological Hallmarks of ALS and Reveal Potential Biomarkers of the Disease in Response to Prolonged IFNγ Exposure

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Figure 10.
Figure 10.

Suppression of CD8+ T-cell proliferation by coculturing with IFNγ-treated neurons. A, Schematic of the cells and antibodies used (left panel). CFSE-labeled CD8+ T-cells were stimulated by incubating them in anti-CD3-coated plates for 5 d, in the absence or presence of IFNγ-treated iPSC-derived neurons. Quantification of CFSE-labeled CD8+ T-cell proliferation is depicted in each histogram (right panels). B, The result summarized in the bar graph showing % of proliferating T-cells.

  1. The Journal of Neuroscience vol. 44 no. 16
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