Transcriptomic profiles of both WT and Q331K^+/− mutant motor neurons are significantly altered by IFNγ treatment. A, Schematic of the single-cell RNA-seq libraries for comparison. B, Volcano plots showing the DEGs detected after IFNγ treatment. The diagram below the volcano plots shows the number of upregulated and downregulated genes for each group after IFNγ treatment. C, A Venn diagram showing the number of ALS-associated DEGs in each group. (D) The GO terms show the functional categories to which the ALS-associated DEGs are targeted. The numbers indicate the number of DEGs included in each GO term.