Table 2.
Summary of quantification for sex-specific 6E10 Aβ immunostaining
| Summary | WT young male | WT young female | WT middle male | WT middle female | 5xFAD young male | 5xFAD young female | 5xFAD middle male | 5xFAD middle female | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| # of mice: 4–5 for each group | ||||||||||||||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
| Density (mm−2) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1,601 | 94.8 | 2,702 | 214.6 | 2,432 | 157.5 | 2,963 | 148.6 |
| Size (μm2) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 68.7 | 2.7 | 74.3 | 2.0 | 130 | 2.9 | 150 | 3.4 |
| Intensity (AU) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1,985 | 36.0 | 3,473 | 37.1 | 3,718 | 37.7 | 3,805 | 48.5 |
| Intracellular density (mm−2) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8,479 | 293.9 | 5,896 | 656.4 | 4,603 | 323.5 | 2,506 | 232.0 |
| Statistical comparison | ||||||||||||||||
| Comparison | 5xFAD young male versus 5xFAD young female | 5xFAD middle male versus 5xFAD middle female | 5xFAD young male versus 5xFAD middle male | 5xFAD young female versus 5xFAD middle female | ||||||||||||
| p value | Significance | p value | Significance | p value | Significance | p value | Significance | |||||||||
| Density (mm−2) | 7.9000 × 10−3 | ** | 5.7100 × 10−2 | ns | 1.5900 × 10−2 | * | 4.1270 × 10−1 | ns | ||||||||
| Size (μm2) | 7.7752 × 10−1 | ns | 2.0914 × 10−1 | ns | 2.6272 × 10−11 | **** | 5.8125 × 10−6 | **** | ||||||||
| Intensity (AU) | 6.7646 × 10−16 | **** | 3.4272 × 10−1 | ns | 1.3916 × 10−23 | **** | 1.1840 × 10−2 | * | ||||||||
| Intracellular density (mm−2) | 3.1700 × 10−2 | * | 7.9000 × 10−3 | ** | 7.9000 × 10−3 | ** | 7.9000 × 10−3 | ** | ||||||||
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The 6E10 amyloid staining quantification results and statistical comparisons summary for both sexes of WT young, WT middle-age, 5xFAD young, and 5xFAD middle-age mice (n = 4–5 for each subgroup). Statistical significances are denoted by *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001. Statistical method: Wilcoxon rank-sum test and LME model.
