NS/NSML transgenes increase synapsin levels with stimulation-dependent loss. A, Representative NMJ synaptic boutons labeled for synapsin (syn, magenta), vesicular glutamate transporter (vglut, green), and HRP (blue) in neuronal transgenic driver control (elav-Gal4/w¹¹¹⁸, left) and driving PTPN11^N308D (elav-Gal4 > PTPN11^N308D, middle) and PTPN11^Q510P (elav-Gal4 > PTPN11^Q510P, right). NMJs without stimulation (basal, top) and 10 min 90 mM [K⁺] (stimulated, bottom). Scale bar: 2.5 µm. Higher-magnification bouton images are shown below. Scale bar: 2.5 µm. B, Quantification of synapsin fluorescence intensity in all six conditions (basal and stimulated). Statistical comparison done using a two-way ANOVA, followed by Tukey’s multiple-comparisons test. C, Quantification of synapsin and vglut colocalization in basal (left) and stimulated (right) conditions using PCC analyses. Statistical comparisons done using one-way ANOVAs followed by Tukey’s multiple-comparisons tests. Scatter plots show all data points with mean ± SEM. Data points: NMJ number. Significance: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), and not significant (n.s.).