Normal synaptic transmission from Ndnf-INs onto Layer 2/3 pyramidal cells in Scn1a+/− mice. A, Schematic of the dual whole-cell patch-clamp recordings of synaptic transmission between Layer 1 Ndnf-INs and Layer 2/3 pyramidal cells in the primary somatosensory cortex. B, C, Representative traces of evoked unitary IPSCs between a presynaptic NGFC and postsynaptic Layer 2/3 pyramidal cell in (B) WT and (C) Scn1a+/− P16–21 mice. Pyramidal cells were held at −50 mV to record outward GABA_A and GABA_B receptor–mediated currents, while Ndnf-INs were stimulated at 0.05 Hz. D, E, Summary data of the (D) amplitude and (E) time to peak of IPSCs show no differences in WT (n = 9 connections from N = 3 mice) versus Scn1a+/− (n = 9 connections from N = 3 mice) Ndnf-INs. All error bars indicate standard error of the mean.