ABC treatment in the mPFC blocks cocaine cue-induced reinstatement when given prior to a novel VR5 memory reactivation session. A, A timeline of experiment. B, Active lever presses during the last 3 d of training were not different among groups. C, The number of cocaine infusions during the last 3 d of training were not different among groups. D, Active lever presses during memory reactivation were higher in the VR5 compared with FR1 groups, with no difference between Veh and ABC groups. E, The number of cocaine infusions during memory reactivation was higher in the FR1 versus pre-VR5 group, with no difference between Veh and ABC groups. F–H, Extinction of active lever presses in FR1, pre-VR5, and post-VR5 groups, respectively. There was decreased responding across the session for all three groups, with no differences between Veh and ABC groups. I, Active lever presses during cue reinstatement. The pre-VR5 ABC group had decreased lever pressing versus the Veh group and versus the post-VR5 ABC group. J, Number of cue rewards during cue reinstatement. The pre-VR5 ABC group had a decreased number of cue rewards versus the Veh group, versus the FR1 ABC group, and versus the post-VR5 ABC group. K, Active lever presses during PR schedule of reinforcement over 3 consecutive days in FR1 and pre-VR5 groups. There was decreased responding in the pre-VR5 versus the FR1 groups across all days. L, M, Microinjection sites for FR1 or pre-VR5 (L) or post-VR5 (M). Gray dots, Veh; red dots, ABC. Data are mean ± SEM. FR1, Veh N = 9; ABC N = 6; pre-VR5, Veh N = 15 (10 for PR); ABC N = 10 (7 for PR); post-VR5, Veh N = 9; ABC N = 7. *p < 0.05. Extended Data Figure 2-1 shows the number of inactive lever presses and rewards from animals shown in Figure 2. Extended Data Figure 2-2 shows statistical comparisons for Figure 2.