ABC treatment in the mPFC prevents increases in beta/gamma power for rewarded lever presses during novel VR5 memory reactivation session. A, Raw and filtered LFP traces from mPFC. B, Spectrograms of mPFC power in the 10–100 Hz range (top panel) and 0–25 Hz range (bottom panel) after rewarded lever presses on the last training day. C, Spectrograms of mPFC power in Veh rats in the 10–100 Hz range (top panel) and 0–25 Hz range (bottom panel) after rewarded lever presses on the VR5 reactivation session. D, Spectrograms of mPFC power in ABC rats in the 10–100 Hz range (top panel) and 0–25 Hz range (bottom panel) after rewarded lever presses on the VR5 reactivation session. For B–D, color bar is relative power. E, Quantification of 60–90 Hz power (top white square in 5C, top panel) in the mPFC 200–600 ms after rewarded lever presses during last day training versus VR5 reactivation session in Veh rats. VR5 reactivation did not alter 60–90 Hz power compared with the last training day. Note that ABC was not delivered until after the last training session, and therefore only Veh animals are shown for the last training day for direct comparison with the Veh group on the VR5 reactivation session. F, Quantification of 30–60 Hz power (middle white rectangle in 5C, top panel) in the mPFC 200–600 ms after rewarded lever presses showed no differences in power compared with the last training day. G, Quantification of 15–30 Hz power (lower white rectangle in 5C, top panel) in the mPFC 200–600 ms after rewarded lever presses showed VR5 reactivation increased 15–30 Hz power compared with the last training day. There were no differences in the 2–4 Hz range at 200–600 ms after rewarded lever presses among groups. H, Quantification of 60–90 Hz power (top white square in 5C, top panel) in the mPFC 200–600 ms after rewarded and unrewarded lever presses. Gamma power was higher in unrewarded versus rewarded lever presses. I, Quantification of 30–60 Hz power (middle white rectangle in 5C, top panel) in the mPFC 200–600 ms after rewarded and unrewarded lever presses. Rewarded lever presses were accompanied by increased power in Veh- versus ABC-treated rats. J, Quantification of 15–30 Hz power (bottom white rectangle in 5C, top panel) in the mPFC 200–600 ms after rewarded and unrewarded lever presses showed no differences between Veh- and ABC-treated rats. K, Location of electrodes in a representative mPFC (top-left panel) and dHIP (top-right panel). Location of all electrodes in the mPFC (bottom-left panel) and location of all electrodes in the hippocampus, with exception of one electrode site that was not identified in one ABC rat (bottom-right panel). Gray dots, Veh; red dots, ABC. Number of rats (number of trials), Veh N = 5 (16 rewarded, 70 unrewarded); ABC N = 3 (17 rewarded, 71 unrewarded). *p < 0.05; ^#p < 0.06. Extended Data Figure 7-1 shows electrode/microinjection sites for Veh- and ABC-treated animals used for in vivo electrophysiology studies shown in Figure 7. Extended Data Figure 7-2 shows statistical comparisons for Figure 7.