Lack of dHIP modulation of mPFC for unrewarded lever presses during novel VR5 memory reactivation, but ABC alters interhemispheric theta/gamma coupling across the mPFC. A, Vehicle rats, comodulogram showing dHIP theta phase and mPFC amplitude 200–600 ms after unrewarded lever presses. B, ABC rats, comodulogram showing dHIP theta phase and mPFC amplitude 200–600 ms after unrewarded lever presses. The color bar is z-score; comodulograms are floored at p < 0.05. C, D, Vehicle (C) and ABC (D) rats, plot of mPFC gamma power (50–70 Hz) binned by dHIP theta phase (8–10 Hz) 200–600 ms after unrewarded lever presses. dHIP theta did not modulate mPFC gamma power in either Veh or ABC rats. E, F, Cross-correlation between mPFC/dHIP theta (4–12 Hz) amplitude envelopes at varying lags in Veh (E) and ABC (F) groups during VR5 memory reactivation session. Stars show peak correlation (mean ± SEM, Veh = 34.5 ± 0.005 ms; ABC = 1.5 ± 0.003 ms). Number of rats (number of trials), Veh N = 4 (59); ABC N = 3 (71). G, Normalized correlation for Neg and Pos lag, with Pos lag indicating mPFC leading the dHIP. There was a decreased correlation in the Veh group, but no lead by mPFC in either group. H, Lag at peak correlation, with a significant difference in distributions between the Veh and ABC groups. I, Veh rats, comodulogram showing left mPFC theta phase and right mPFC amplitude 200–600 ms after unrewarded lever presses. J, ABC rats, comodulogram showing left mPFC theta phase and right mPFC amplitude 200–600 ms after unrewarded lever presses. The color bar is z-score; comodulograms are floored at p < 0.05. K, Vehicle rats, plot of mPFC gamma power (70–90 Hz) binned by dHIP theta phase (8–10 Hz) 200–600 ms after unrewarded lever presses. Left mPFC theta significantly modulates right mPFC gamma power. L, ABC rats, the plot of mPFC gamma power (70–90 Hz) binned by dHIP theta phase (8–10 Hz) 200–600 ms after unrewarded lever presses. Left mPFC theta does not modulate right mPFC gamma power after lever presses. M, Veh rats, the plot of mPFC gamma power (70–90 Hz) binned by dHIP theta phase (5–7 Hz) 200–600 ms after unrewarded lever presses. Left mPFC theta does not modulate right mPFC gamma power. N, ABC rats, the plot of mPFC gamma power (70–90 Hz) binned by dHIP theta phase (5–7 Hz) 200–600 ms after unrewarded lever presses. Left mPFC theta modulates right mPFC gamma power. O, P, Cross-correlation between left mPFC/right mPFC theta (4–12 Hz) amplitude envelopes at varying lags in Veh (O) and ABC (P) groups. Stars show peak correlation (mean ± SEM, Veh = −5.5 ± 0.004 ms; ABC = −4.5 ± 0.003 ms). The line is mean; shading represents SEM. Q, Normalized correlation for Neg and Pos lag, with Pos lag indicating left mPFC leading the right mPFC. There was no effect of ABC treatment. R, Lag at peak correlation, with no difference in distributions between Veh and ABC groups. The box plot shows median and 95% confidence intervals. *p < 0.05. Extended Data Figure 9-1 shows statistical comparisons for Figure 9.