Table 1.
Key resources
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit anti-VapB | ProteinTech | 14477-1-AP |
| Mouse anti-acetylated tubulin | Sigma-Aldrich | T7451 |
| Anti-Rabbit-HRP | Jackson ImmunoResearch Laboratories | 711-035-152 |
| Goat anti-digoxigenin-AP | Roche | 11266027 |
| Chicken anti-GFP | Aves | GFP-1020 |
| Rabbit anti-SSBP1 | ProteinTech | 12212-1-AP |
| Chemicals, peptides, and recombinant proteins | ||
| Cas9 protein | Integrated DNA Technologies | 1081058 |
| Critical commercial assays | ||
| Custom HCR in situ hybridization probe set and buffer | Molecular Instruments | N/A |
| SuperScript IV First-Strand Synthesis System | Thermo Fisher Scientific | 18091050 |
| QuikChange II Site-Directed Mutagenesis Kit | Agilent Technologies | 200523-5 |
| Experimental models: cell lines | ||
| HEK293T | ATCC | CRL-3216 |
| Experimental models: organisms/strains | ||
| Zebrafish AB strain; 4 dpf; sex not determined at this stage | ZIRC | ZL1 |
| Zebrafish mitfaw2 (nacre) strain; 4 dpf; sex not determined at this stage | ZIRC | ZL2104 |
| Zebrafish TgBAC(neurod:eGFP)nl1; 4 dpf; sex not determined at this stage | ZIRC | ZL1747 |
| Zebrafish Tg(-5kbneurod1:pgc1α-p2a-mRFP)uwd9; 4 dpf; sex not determined at this stage | This paper | ZFIN: ZDB-ALT-231026-11 |
| Zebrafish vapbuwd6; 4 dpf; sex not determined at this stage | This paper | ZFIN: ZDB-ALT-231023-16 |
| Recombinant DNA | ||
| Plasmid: -5kbneurod1:VapB-HaloTag | This paper | N/A |
| Plasmid: -5kbneurod1:VapBP56S-HaloTag | This paper | N/A |
| Plasmid: -5kbneurod1:VapB-eGFP | This paper | N/A |
| Plasmid: -5kbneurod1:VapBP56S-eGFP | This paper | N/A |
| Plasmid: -5kbneurod1:VapB-p2a-TagBFP | This paper | N/A |
| Plasmid: -5kbneurod1:VapBP56S-p2a-TagBFP | This paper | N/A |
| Plasmid: -5kbneurod1:mito-eGFP | This paper | N/A |
| Plasmid: -5kbneurod1:mito-tagRFP | (Drerup et al., 2017) | N/A |
| Plasmid: -5kbneurod1:Tomm20-Halotag | This paper | N/A |
| Plasmid: -5kbneurod1:RFP-LC3 | (Drerup and Nechiporuk, 2013) | N/A |
| Plasmid: -5kbneurod1:mito-GFP-mCherry | (Rojansky et al., 2016) | Parent plasmid: Addgene 78520 |
| Plasmid: -5kbneurod1:Tomm20-Halotag-sec61β | This paper | Parent plasmid: Addgene 141152 |
| Plasmid: -5kbneurod1:SPLICS | (Cieri et al., 2018) | Parent plasmids: Addgene 164121, 164119 |
| Plasmid: -5kbneurod1:eGFP | This paper | N/A |
| Plasmid: -5kbneurod1:mito-tagRFP-p2a-gfpCAAX | This paper | N/A |
| Software and algorithms | ||
| ImageJ/Fiji software | (Schindelin et al., 2012) | RRID:SCR_002285 |
| Metamorph software | Molecular Devices | RRID:SCR_002368 |
| Prism 9 | GraphPad | RRID:SCR_002798 |
