Table 4.
Acquisition setting ranges used for VapB and VapBP56S imaging
| Laser power | HV | |||
|---|---|---|---|---|
| Min | Max | Min | Max | |
| VapB-eGFP (live) in Figures 1F,H, 2A–G | 2.8 | 3 | 550 | 700 |
| VapBP56S-mRFP (live) in Figures 1G,H, 2A–G | 3 | 3 | 500 | 700 |
| VapB-HaloTag in Figures 1C, 3C,G,H, 4A–E, 6B,D–F,H,J | 2.1 | 3 | 620 | 700 |
| VapBP56S-HaloTag in Figures 1D, 3D,G,H, 4A–E, 6C,D–F,I,J | 2.3 | 3 | 580 | 700 |
| VapB-p2a-TagBFP in Figure 3E,G | 0.8 | 1.2 | 610 | 700 |
| VapBP56S-p2a-TagBFP in Figure 3F,G | 0.8 | 1.6 | 610 | 700 |
| VapB-eGFP (fixed; HCR) in Figure 1J,L | 2.8 | 3 | 600 | 700 |
| VapBP56S-eGFP (fixed; HCR) in Figure 1K,L | 3 | 3 | 630 | 700 |
| VapB-eGFP (anti-GFP; immunostaining) in Figure 1N,P | 0.07 | 1.8 | 450 | 560 |
| VapBP56S-eGFP (anti-GFP; immunostaining) in Figure 1O,P | 0.4 | 3 | 460 | 700 |
