Table 3.
Experimental design and statistics: list of statistical tests, test statistics, and p values for all experiments in the study
| Figure | Experiment | Strains | Sample size: N (censored) | Number of WorMotels | Statistical test | Test statistic | p value | Post hoc test | Post hoc test statistic and p value |
|---|---|---|---|---|---|---|---|---|---|
| 1c | WorMotel movement | WM27 | 73(15), 122(78) | 12 | Unpaired t test | t(191) = 9.64 | <0.0001 | – | – |
| 1d | WorMotel bout duration | WM27 | 73(15), 122(78) | 12 | Unpaired t test | t(191) = 8.36 | <0.0001 | – | – |
| 1e | WorMotel bout number | WM27 | 73(15), 122(78) | 12 | Unpaired t test | t(191) = 10.43 | <0.0001 | – | – |
| 1f | Feeding | WM27 | 41(0), 47(0) | – | Unpaired t test | t(86) = 5.37 | <0.0001 | – | – |
| 1g | Movement + Feeding | WM27 | 155(0) | – | Chi-square | X(1) = 63.52 | <0.0001 | – | – |
| 2a | Mechanical reversibility | WM27 | 12(30) (repeated measures) | – | Paired t test | t(11) = 12.35 | <0.0001 | – | – |
| 2b | Mechanical stim response probability | WM27 | 35(6) | 1 | Restricted maximum likelihood model | F(1.58,28.4) = 22.6 | <0.0001 | Šídák | SHAM vs STIM: active t(34) = 6.03, p < 0.0001; qui t(11) = 1.24, p = 0.4239 |
| 2c | Octanol response latency | WM27 | 17(0), 32(0) | – | Unpaired t test | t(47) = 6.86 | <0.0001 | – | – |
| 3c | Homeostasis (change from baseline) | WM27 uninfected | Sham: 6(2); stim: 9(7) | 3 | 2-way mixed ANOVA | Interaction effect F(1,14) = 0.003 | p = 0.957 | – | – |
| WM27 infected | Sham: 20(19); stim: 40(39) | 3 | 2-way mixed ANOVA | Interaction effect F(1,58) = 12.73 | p = 0.0007 | Šídák | STIM-PRE: t(116) = 3.49, p = 0.0014; POST-PRE: t(116) = 0.399, p = 0.904 | ||
| 3d | Homeostatic (bouts duration) | WM27 uninfected | Sham: 6(2); stim: 9(7) | 3 | Restricted maximum likelihood model | Interaction effect F(1,24) = 0.898 | p = 0.353 | – | – |
| WM27 infected | Sham: 20(19); stim: 40(39) | 3 | 2-way mixed ANOVA | Interaction effect F(1,58) = 1.29 | p = 0.261 | – | – | ||
| 3e | PRQ comparison | WM27 | 6(0) and 36(5) | 1 | 2-way mixed ANOVA | Interaction effect: F(1,40) = 10.42 | p = 0.002 | Šídák | SHAM vs STIM: uninfected t(40) = 1.99, p = 0.103; infected t(40) = 3.66, p = 0.001 |
| 4b | UV WorMotel: ceh-17, aptf-1, egl-21 | WM27, NQ1167, NQ1183, NQ1184 | 10(0), 7(1), 9(1), 8(0) (repeated measures) | 1 | Restricted maximum likelihood model | Interaction F(3,30) = 28.96 | p < 0.0001 | Dunnett | Untreated (vs WM27): NQ1167 q(61) = 0.1623 p = 0.99; NQ1183 q(61) = 0.098 p = 0.99; NQ1184 q(61) = 0.21 p = 0.99; UV (vs WM27): NQ1167 q(61) = 6.96 p < 0.0001; NQ1183 q(61) = 12.26 p < 0.0001; NQ1184 q(61) = 9.41 p < 0.0001 |
| 4c | Orsay WorMotel: egl-21 | WM27, NQ1184 | 14(0), 14(0), 24(3), 25(2) | 2 | 2-way ANOVA | Interaction effect: F(1,73) = 15.48 | p = 0.0002 | Šídák | Uninfected t(73) = 0.130, p = 0.9894; infected, t(73) = 6.695, p < 0.0001 |
| 4d | Orsay WorMotel: ceh-17 | WM27, NQ1167 | 31(1), 32(0), 25(7), 30(2) | 3 | 2-way ANOVA | Interaction F(1,114) = 16.42 | p < 0.0001 | Šídák | Uninfected t(114) = 0.2056, p = 0.9736; infected t(114) = 5.343 p < 0.0001 |
| 4e | Orsay WorMotel: ceh-14 | WM27, NQ1230 | 4(0), 4(0), 20(0), 20(0) | 1 | 2-way ANOVA | Interaction F(1,44) = 1.17 | p = 0.286 | Šídák | Uninfected t(44) = 0.016, p = 0.999; infected t(44) = 2.68, p < 0.020 |
| 4f | Orsay WorMotel: aptf-1 | WM27, NQ1183 | 24(8), 27(5), 36(28), 35(29) | 4 | 2-way ANOVA | Interaction F(1,118) = 0.4435 | p = 0.5068 | Šídák | Uninfected t(118) = 0.5134 p = 0.8468; infected t(118) = 0.4242, p = 0.8925 |
| 4g | Orsay WorMotel: flp-11 | WM27, NQ1251 | 31(1), 28(4), 47(17), 40(24) | 4 | 2-way ANOVA | Interaction F(1,142) = 2.92 | p = 0.0899 | Šídák | Uninfected t(142) = 0.069 p = 0.997; infected t(142) = 2.6, p = 0.0205 |
| 4h | UV WorMotel: RIS HisCl | WM27, NQ1231 | 2(2), 3(1), 5(7), 9(3) | 3 | 2-way ANOVA | Interaction F(1,81) = 27.58 | p = 0.0001 | Šídák | Untreated t(81) = 0.064 p = 0.99; UV t(81) = 8.60 p < 0.0001 |
| 4i | Orsay WorMotel: RIS HisCl | No his WM27, NQ1231 | 8(0), 6(2), 9(7), 10(6) | 1 | 2-way ANOVA | Interaction F(1,29) = 0.007 | p = 0.934 | Šídák | Uninfected t(29) = 0.104 p = 0.99; infected t(29) = 0.251 p = 0.96 |
| 10 mM his WM27, NQ1231 | 63(7), 62(14), 80(36), 65(55) | 8 | 2-way ANOVA | Interaction F(1,266) = 9.27 | p = 0.0026 | Šídák | Uninfected t(266) = 0.527 p = 0.839; infected t(266) = 3.88 p = 0.0002 | ||
| 5a | Orsay pumping | WM27, NQ1167, NQ1230, NQ1183, NQ1184 | 44(0), 50(0), 50(0), 28(0), 38(0); 50(0), 50(0), 40(0), 29(0), 44(0) | – | 2-way ANOVA | Interaction F(4,413) = 10.67 | p < 0.0001 | Dunnett | Uninfected (vs WM27): NQ1167 q(413) = 0.97 p = 0.737; NQ1230 q(413) = 0.17 p = 0.999; NQ1183 q(413) = 2.65 p = 0.030; NQ1184 q(413) = 2.49 p = 0.046; Orsay (vs WM27): NQ1167 q(413) = 5.67 p < 0.0001; NQ1230 q(413) = 5.48 p < 0.0001; NQ1183 q(413) = 1.26 p = 0.545; NQ1184 q(413) = 3.75 p = 0.0008 |
| 5b | UV pumping | WM27, NQ1167, NQ1230, NQ1183, NQ1184 | 40(0), 30(0); 25(0), 20(0); 25(0), 20(0); 24(0), 20(0); 30(0), 30(0) | – | 2-way ANOVA | Interaction F(4,254) = 70.25 | p < 0.0001 | Dunnett | Untreated (vs WM27): NQ1167 q(254) = 1.16 p = 0.631; NQ1230 q(254) = 1.42 p = 0.450; NQ1183 q(254) = 1.12 p = 0.656; NQ1184 q(254) = 0.49 p = 0.988; UV (vs WM27): NQ1167 q(254) = 7.11 p < 0.0001; NQ1230 q(254) = 20.30 p < 0.0001; NQ1183 q(254) = 0.05 p = 0.999; NQ1184 q(254) = 0.10 p = 0.999 |
| 6a–d | Survival | see Tables 4–7 | |||||||
| 6f | FLP-13 OE WorMotel | WM27, NQ1292 | 4(0), 3(1), 19(1), 19(1) | 1 | 2-way ANOVA | Interaction F(1,41) = 3.34 | p = 0.075 | Šídák | 20°C t(41) = 0.008 p > 0.99; 25°C t(41) = 4.69 p < 0.0001 |
| 7a | Orsay virus load | WM27, NQ1167 | 12(0), 12(0) | – | Mann–Whitney test | U = 60 | p = 0.514 | – | – |
| 7b | cul-6 | WM27, NQ1167 | 3(1), 3(1), 4(1), 4(1) | – | Multiple 2-way ANOVA: family-wise alpha 0.05 (Bonferroni’s corrected to 0.007) | Infection F(1,10) = 198.2; interaction F(1,10) = 1.10 | p < 0.0001; p = 0.32 | ||
| F26F2.1 | 3(1), 3(1), 4(1), 4(1) | – | Infection F(1,10) = 3,290; interaction F(1,10) = 0 | p < 0.0001; p > 0.99 | |||||
| F26F2.3 | 4(0), 4(0), 5(0), 5(0) | – | Infection F(1,14) = 459.4; interaction F(1,14) = 1.13 | p < 0.0001; p = 0.20 | |||||
| F26F2.4 | 2(2), 2(2), 3(2), 3(2) | – | Infection F(1,6) = 632.2; interaction F(1,6) = 4.74 | p < 0.0001; p = 0.07 | |||||
| pals-5 | 4(0), 4(0), 5(0), 5(0) | – | Infection F(1,14) = 238.8; interaction F(1,14) = 0.92 | p < 0.0001; p = 0.35 | |||||
| skr-4 | 3(1), 3(1), 4(1), 4(1) | – | Infection F(1,10) = 74.8; interaction F(1,10) = 0.42 | p < 0.0001; p = 0.42 | |||||
| skr-5 | 3(1), 4(0), 5(0), 5(0) | – | Infection F(1,13) = 55.0; interaction F(1,13) = 1.19 | p < 0.0001; p = 0.30 | |||||
| 7c | GFP bacteria | WM27, NQ1167 | See Table 8 (all between subjects comparisons) | ||||||
| 7d | Dead bacteria survival | WM27 | See Table 9 | ||||||
| 7e | ATP | WM27, NQ1167 | 3(0), 7(0), 8(0), 8(0) | – | 2-way ANOVA | Interaction F(1,22) = 14.7 | p = 0.0009 | Šídák | WM27, t(22) = 1.99, p = 0.11; NQ1167, t(22) = 9.28, p < 0.0001 |
-
Sample sizes (N) are shown for each group in the order that they appear in the figures; the number of censored animals for each group is shown next to the sample size in parentheses.
