Expression levels of key molecular markers are not different in RTN neurons from WT and GPR4(H81F) mice. A, B, Single-cell qRT-PCR results for RTN markers Nmb and vGlut2 as well as for proton sensors Gpr4 and Kcnk5 from adult (A, P49–P109) and neonatal (B, P7–P11) RTN neurons (adult: WT Jx99, N = 4; n = 27; H81F-Jx99, N = 3; n = 20; neonate: WT Jx99, N = 3; n = 24; H81F-Jx99, N = 3; n = 28; unpaired t test).