The slow EPSC (sEPSC) was heterogenous across cerebellar lobules. a, Schematic representation of a mouse brain highlighting the recorded regions: the cerebellar flocculus and vermis. Parasagittal acute cerebellum slices were used for recordings in vermis lobules IV/V and X, while coronal sections were used for flocculus recordings. b, Representative sEPSC traces depict a delayed time of peak in lobule X and the flocculus compared with lobules IV/V. Scale bar, 100 pA, 500 ms. c, Peak amplitudes of sEPSCs were similar across lobules. *p < 0.05, Kruskal–Wallis analysis of variance on ranks followed by Dunn's multiple-comparisons test. d, sEPSC time of peak was earlier in lobules IV/V in comparison with lobule X and the flocculus. Kruskal–Wallis analysis of variance on ranks followed by Dunn's multiple-comparisons test (****p < 0.0001). e, High heterogeneity in the time of peak within lobule X and the flocculus compared with lobules IV/V is demonstrated by higher coefficients of variation. f, Lobule X sEPSCs exhibited the longest 90–10% decay time. Both lobule X and flocculus sEPSCs had longer decay times than those from lobules IV/V. *p < 0.05, ****p < 0.0001, Kruskal–Wallis analysis of variance on ranks followed by Dunn's multiple-comparisons test. g, A longer half-width sEPSC was observed in lobule X and the flocculus compared with lobules IV/V (****p < 0.0001, Kruskal–Wallis analysis of variance on ranks followed by Dunn's multiple-comparisons test). h, sEPSCs with similar peak amplitudes in lobules IV/V, lobule X, and the flocculus had different times of peak. Bubble size represents the decay time of the sEPSC. Data from each lobule were fit in an assumption-free manner with a spline in equivalent color. Violin plots show median and quartiles. (c–f, h: lobules IV/V = 56 cells from 41 mice, lobule X = 35 cells from 27 mice, flocculus = 23 cells from 14 mice. g: lobules IV/V = 42 cells from 33 mice, lobule X = 33 cells from 26 mice, flocculus = 23 cells from 14 mice). Please refer to Extended Data Figures 1-1–1-3 for further information. Additional details on statistics are presented in Materials and Methods and Extended Data Table 1.