Heterogeneity in Slow Synaptic Transmission Diversifies Purkinje Cell Timing

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Figure 4.
Figure 4.

Heterogeneity of sEPSC properties persisted across a range of PF stimulation frequencies. a, From left to right, Peak amplitude of sEPCS in lobules IV/V, lobule X, and the flocculus are similar, in response to 10 PF stimulations at 50, 75, 100, 125, 150, and 200 Hz. **p < 0.01, Kruskal–Wallis test followed by Dunn's multiple-comparisons test. b, From left to right, sEPSC time of peak is earlier in lobules IV/V in comparison with lobule X and the flocculus, in response to 10 PF stimulations at 50, 75, 100, 125, 150, and 200 Hz. ****p < 0.0001, Kruskal–Wallis test followed by Dunn's multiple-comparisons test. c, From left to right, Time for the sEPSC peak amplitude to decay from 90 to 10% was longer for both lobule X and the flocculus in comparison with lobules IV/V, in response to 10 PF stimulations at 50, 75, 100, 125, 150, and 200 Hz. 50 Hz, 75 Hz *p < 0.05, ****p < 0.0001, Kruskal–Wallis test followed by Dunn's multiple-comparisons test. 100–200 Hz ***p < 0.001, ****p < 0.0001, ordinary one-way ANOVA followed by Tukey's multiple-comparisons test. In order to compare the effect of PF stimulation frequency on the sEPSC, the 100 Hz dataset included a subset of the cells that contributed to Figure 1, as well as to Figure 9. Violin plots show median and quartiles. (50 Hz: lobules IV/V = 24 cells from 18 mice, lobule X = 17 cells from 17 mice, flocculus = 8 cells from 6 mice; 75 Hz: lobules IV/V = 26 cells from 18 mice, lobule X = 17 cells from 17 mice, flocculus = 8 cells from 6 mice; 100 Hz: lobules IV/V = 28 cells from 18 mice, lobule X = 17 cells from 17 mice, flocculus = 8 cells from 6 mice; 125 Hz: lobules IV/V = 25 cells from 18 mice, lobule X = 17 cells from 17 mice, flocculus = 8 cells from 6 mice; 150 Hz: lobules IV/V = 25 cells from 17 mice, lobule X = 17 cells from 17 mice, flocculus = 8 cells from 6 mice; 200 Hz: lobules IV/V = 24 cells from 16 mice, lobule X = 16 cells from 16 mice, flocculus = 8 cells from 6 mice). Additional details on statistics are in Extended Data Table 1.

  1. The Journal of Neuroscience vol. 44 no. 37
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