sEPSCs exhibited equivalent mGluR and TRPC3 dependence across lobules. a, Top, Representative sEPSC traces from lobules IV/V, lobule X, and the flocculus. Colored traces are sEPSCs at baseline, and gray traces represent sEPSCs after blockade by wash-in of noncompetitive mGluR1 antagonist CPCCOEt (100 µM). Bottom, sEPSCs were blocked by wash-in of CPCCOEt. ****p < 0.0001, paired t test. b, c, Top, Representative sEPSC traces from lobules IV/V, lobule X, and the flocculus. Colored traces are sEPSCs at baseline, and gray traces are sEPSCs after wash-in. Bottom, Wash-in of (b) genistein (100 µM) or (c) SKF96365 (10 µM) reduced sEPSC amplitude across all cerebellar lobules tested. **p < 0.01, ***p < 0.001, Wilcoxon matched-pairs signed rank test. d, e, Partial blockade of TRPC3 did not explain heterogeneity in time of peak across lobules. Statistics were not run to compare different lobules as the number of cells in each lobule was too low. Scale bar, 100 pA, 0.5 s. Violin plots show median and quartiles. (a: lobules IV/V = 4 cells from 3 mice, lobule X = 5 cells from 3 mice, flocculus = 3 cells from 2 mice. b, d: lobules IV/V = 3 cells from 2 mice, lobule X = 3 cells from 2 mice, flocculus = 3 cells from 3 mice. c, e: lobules IV/V = 5 cells from 4 mice, lobule X = 3 cells from 3 mice, flocculus = 3 cells from 3 mice). Additional details on Statistics are in Extended Data Table 1.