TRPC3 to mGluR1a ratio was higher in lobules IV/V, but mGluR1 and TRPC3 localization was the same across lobules. a, b, Antibody staining for calbindin, mGluR1a, and TRPC3 on 50-µm-thick cerebellar sections demonstrates similar cellular localization across lobules. Scale bars: a, 100 µm; b, 50 µm. c, Mean fluorescence intensities of TRPC3 and mGluR1a were measured in the molecular layer of each lobule, the background intensity was subtracted, and the ratio of TRPC3 to mGluR1 was determined. Each data point is one region from one brain section. For each region (lobules IV/V, lobule X, flocculus), there are two sections per mouse and four mice per region. One-way ANOVA followed by Tukey's multiple-comparisons test, ****p < 0.0001.