sEPSC heterogeneity diversifies Purkinje cell spike timing. a–c, sEPSC traces in response to 10 PF stimulations at 100 Hz recorded from Purkinje cells voltage-clamped at −70 mV. Scale bar 100 pA, 250 ms. d–f, Mean spike counts binned into 100 ms time bins from the same Purkinje cells, spiking in current-clamp conditions, in response to the same stimulation of 10PF stimulations at 100 Hz. g–i, Overlay of 100 ms time-binned mean spike counts from the same Purkinje cells under current-clamp conditions in response to 10PF stimulations at (g) 50 Hz, (h) 100 Hz, and (i) 150 Hz. j, Top, Representative trace of a cell-attached recording from lobules IV/V, with PF stimulation at time 0 and 10 stimulus pulses at 100 Hz. Bottom, Normalized instantaneous spike frequency binned into 100 ms time bins. The representative cell-attached trace is aligned in time to the x-axis of the graph. k, l, Same as in j but for lobule X and the flocculus, respectively. All data are mean ± SEM. (a–i: lobules IV/V = 4 cells from 4 mice, lobule X = 8 cells from 8 mice, flocculus = 5 cells from 4 mice; j–l: lobules IV/V = 11 cells from 4 mice, lobule X = 8 cells from 3 mice, flocculus = 9 cells from 3 mice).