Changes in subtypes of ENS neurons in the adult Tuba1a-Cre;Elp1^loxp/loxp cKO mice. A, B, Whole mounts of ileum were immunolabeled with antibodies to (A) ChAT (green) and to neurons (Hu, magenta); cholinergic neurons in the myenteric plexus are increased in the adult Chat-GFP;Tuba1a-Cre;Elp1^LoxP/LoxP cKO myenteric plexus (multiple unpaired t tests; *p = 0.0402; ns = 0.075; n = 3) while (C, D) the percentage of dopaminergic neurons is reduced in the submucosal plexus in the Tuba1a-Cre;Elp1^loxp/loxp cKO whole mounts. Tyrosine hydroxylase, red; neurons (Hu, green); multiple unpaired t tests; *p = 0.0154; n = 4.