Analysis of Ki67+ and Sox2+ cells in the adult Tuba1a-Cre;Elp1^loxp/loxp cKO ileum. A–C, Whole mounts were labeled with antibodies to Ki67 (green, A) and the neuronal marker Hu (magenta) and the number of positive cells quantified (B, C); B, unpaired t test p value indicated in graph, n = 4 (C) unpaired t test p value indicated in graph, n = 4. D–F, Whole mounts were immunolabeled with antibodies to Hu (green) and Sox2 (red) the number of Sox2+ cells determined (E) unpaired t test p value indicated in graph. Asterisks (*) indicate Sox2+ intraganglionic cells. N = 4 mice in each genotype group; in each experiment, one Tuba1a-Cre;Elp1^loxp/loxp cKO was compared with one control which were immunolabeled and imaged together. However, due to interexperiment variation (Fig. 9E), when pooled together, the differences between cKO and control are not significant. However, given this variation between experiments, if one compares the Tuba1a-Cre;Elp1^loxp/loxp cKO to the control within each experiment, there was a consistent and significant reduction in Sox2+ cells in the cKO compared with the control (Fig. 9F). F, Lines connect the number of Sox2+ cells between the control and cKO mouse in the same experiment to indicate the consistent relative reduction in Sox2+ cells in the cKO compared with the control across all 4 mouse pairs (paired t test p = 0.026).