ELP1, the Gene Mutated in Familial Dysautonomia, Is Required for Normal Enteric Nervous System Development and Maintenance and for Gut Epithelium Homeostasis

Table 1.

Primary antibodies

Antigen Host species Dilution Source
HuC/D ANNA1 serum Human 1:20,000 Kind gift from Dr Vanda Lennon, Mayo Clinic, Rochester, MN, USA
eGFP Chick 1:2,000 Abcam, ab13970
Serotonin Rat 1:200 Abcam, ab6336
CGRP Rabbit 1:400 Millipore, PC205L
Ki67 Rabbit 1:400 EMD Millipore SAB5500134
Elp1/Ikap Rabbit 1:400 Sigma, SAB2701068
Tyrosine hydroxylase Rabbit 1:100 Millipore, AB152
VIP Rabbit 1:750 ImmunoStar, 20077
Cleaved caspase-3 Rabbit 1:250 Cell Signaling, 9664
Synapsin Rabbit 1:200 Cell Signaling, D12G5
CD117 Goat 1:300 LS-B16042-50
S100b Rabbit 1:400 Dako, GA504
E-cadherin Mouse 1:50 BD Transduction Laboratories, 610181
CAI (CAR-1) Rabbit 1:200 Proteintech, 13198-2-ap
Muc2 Rabbit 1:100 LifeSpan BioSciences, LS-C295438
  1. The Journal of Neuroscience vol. 44 no. 37
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