Immunofluorescence images of postnatal mouse dLGN stained with antibodies to NP1 (first column), NP2 (second column), or NPR (third column) at P7 or P14. A–F, WT retinas. G–I, NP1/2 KO retinas. A, D, NP1 is expressed in dLGN neurons at P7 and P14 but with relatively lower levels at P14. B, E, NP2 is expressed on axons projecting to the dLGN (see Results). B, At P7, axonal expression is present throughout the dLGN except for in the innermost segment (asterisk), whereas at P14 (E), NP2 expression is enriched in the outer segment of the dLGN. C, F, Low levels of NPR are present at P7 and P14. G–I, No specific label is present for NP1, NP2, or NPR in dLGNs from NP1/2 KO mice, and NPR levels are reduced in NP1/2 KO mice relative to WT mice. Hipp, Hippocampus; IGL, intergeniculate leaflet; VPL, ventral posterolateral nucleus. Coronal plane is shown; dorsal is up and medial is to the right of each panel. The boundary of the dLGN is shown with the dashed line. Scale bar, 200 μm.