Depletion of MTCL2 resulted in the development of fewer and longer dendrites. A, Full views of single CGNs treated with the indicated RNAi and fixed at DIV6. The cells were visualized using GFP signal. Scale bar: 50 µm. B, Distribution of the total axonal length of individual CGNs subjected to the indicated RNAi and fixed at DIV6. C, Dendritic development of CGNs subjected to the indicated RNAi and fixed at DIV6. Cells were visualized using GFP and MAP2 signals. The arrowheads indicate the dendrites of GFP-positive cells. Scale bar, 10 µm. D, Distribution of the total number of dendritic tips of individual CGNs subjected to the indicated RNAi/rescue conditions and fixed at DIV6. E, Distribution of the averaged dendritic lengths of the individual CGNs analyzed in D. Each data point in B, D, and E represents the result of one neuron from three independent experiments. The means ± SD are shown for each scatterplot. F, The percentage of dendrites longer than 40 µm in all dendrites of the same CGNs analyzed in D and E. Each data point represents the average value from each of the three independent experiments. The means ± SE are shown. For all datasets, statistical significance was assessed using the Kruskal–Wallis test with the Holm-corrected Mann–Whitney U test, except for F, for which a one-way analysis of variance test was employed in conjunction with a Holm-corrected Welch's two-sample t test.