Dendrite extensions and Golgi apparatus positions of MTCL2-depleted CGNs biased in the direction opposite to axonal elongation. A, The extension directions of dendrites longer than 15 µm were defined as the angle (θ) formed between the roots of the dendrite and an axon, as depicted. B, θ distribution of individual dendrites of CGNs subjected to the indicated RNAi/rescue conditions and fixed at DIV6. The θ values are categorized in four directions and presented in polar histograms of the average values from each of the three independent experiments (more than 350 dendrites from the indicated number of cells were analyzed for each condition). Data represent the means ± SE. C, Comparison of dendritic direction bias (r) between the conditions. r = 1 indicates no direction bias. Each data point represents the average value from each of the three independent experiments. The means ± SE are shown. D, The position of the Golgi apparatus for individual CGNs was estimated as the angle (θ) formed between the center of the Golgi apparatus and the roots of an axon, as depicted. E, θ distribution of the Golgi position in CGNs subjected to the indicated RNAi/rescue conditions and fixed at DIV6. The θ values are categorized in four directions and presented in polar histograms of the average values for each from the three independent experiments (indicated number of cells were analyzed for each condition). Data represent the means ± SE. F, Comparison of direction bias (r) of the Golgi position under each condition. Each data point represents the average value from each of the three independent experiments. The means ± SE are shown. In C and F, the one-way ANOVA test with the Holm-corrected Welch's two-sample t test was employed to ascertain statistical significance.