The Golgi apparatus in differentiated CGNs was sequestered into the MT cage surrounding the nucleus. A, High-resolution confocal images of mature CGNs (DIV5) stained for tyrosinated tubulin, MTCL2, and GS28 (Golgi membrane protein). Three images of the same field in different confocal planes are presented from the basal to apical planes, as indicated. Scale bar, 5 µm. B, The boxed regions in A were enlarged to show the localization of tyrosinated tubulin, MTCL2, and the Golgi apparatus. The orange arrows indicate the typical sites where MTCL2 signals localize to the contact sites between the MT cage and the Golgi apparatus. Scale bar, 5 µm. C, Cross-sectional views of the z-stack images along the dotted line in A. Two types of merged images with different antibody combinations are presented. The arrows indicate the Golgi apparatus at the top of the nucleus. Scale bar, 2 µm. D, High-resolution confocal images of immature CGNs at DIV2 and DIV3 after staining with tyrosinated tubulin and GS28. Scale bar, 5 µm. E, Height distribution of the apical edge of the Golgi apparatus estimated as a percentage of the cell heights (Fig. S7A). Each data point represents the result of one neuron observed in three independent experiments. The means ± SD are shown for each scatterplot. The Kruskal–Wallis test was employed in conjunction with the Holm-corrected Mann–Whitney U test to ascertain statistical significance.