MTCL2 promotes the upward extension of the Golgi apparatus in an MT- and Golgi-binding activity-dependent manner. A, High-resolution confocal images of CGNs subjected to the indicated RNAi, fixed at DIV6, and stained for tyrosinated tubulin and GS28. On the left, the projected views of the z-stack images are shown to indicate cells expressing the indicated shRNA vector together with GFP. On the right, the confocal images obtained at three different focus planes are shown. Scale bar, 5 µm. B, Cross-sectional views of the z-stack images along the dotted lines in A. Asterisks indicate GFP-positive cells. Scale bar: 5 µm. C, Height distribution of the Golgi apical edge in CGNs subjected to the indicated RNAi/rescue conditions and fixed at DIV6. Each data point represents the result of one neuron observed in three independent experiments. The means ± SD are shown for each scatterplot. The Kruskal–Wallis test was employed in conjunction with the Holm-corrected Mann–Whitney U test to ascertain statistical significance. D, Comparison of MT cage density in CGNs subjected to different conditions. The intensity of the tyrosinated tubulin signal across the entire cell body of a single CGN was quantified along the eight lines shown above and averaged for each CGN based at each normalized distance from the cell center. The results were further averaged for cells obtained from two independent experiments and plotted from the cell center to the periphery according to the normalized distance. The number of cells (n) analyzed for each condition is indicated in parentheses. The means ± SD are shown. The p values estimated using the one-way ANOVA test with the Holm-corrected Welch’s two-sample t test are listed in Figure S7F.