Experimental design. A, Viral vector and optic fiber stub targeting strategy for optogenetic manipulation of the central amygdala (CEA) in CRF-Cre and SOM-Cre mice. Partially created with BioRender.com. B, Representative images of EYFP-tagged virus expression (green) in the CEA and fiber stub implantation above the CEA in CRF-Cre (left) and SOM-Cre (right) mice. Scale bar represents 200 µm. C, Graphic representation of the serial compound stimulus (SCS) and timing of the light delivery for optogenetic manipulation during white noise presentation. D, Stages of the conditioned flight paradigm with optogenetic manipulation over 5 d. Colored squares indicate the day mice receive light through attached fiberoptic patch cables. E, Graphic collection of viral expression (green ovals) and fiber placements (magenta rectangles) of each CRF-Cre group in the CEA, distributed through coronal brain sections. F, Graphic collection of viral expression (green ovals) and fiber placements (magenta rectangles) of each SOM-Cre group in the CEA, distributed through coronal brain sections. CRF, corticotropin-releasing factor; SOM, somatostatin.