FISH analysis of colocalization of CRF+ and SOM+ CEA neurons. A, Viral vector targeting strategy for FISH of the CEA in CRF-Cre and SOM-Cre mice. Partially created with BioRender.com. B, Representative images of the CEA 3 weeks postinjection in CRF-Cre and SOM-Cre mice. Images above and below dotted line represent CRF-Cre and SOM-Cre groups, respectively. Biomarkers stained via FISH and immunohistochemistry are nuclei (blue), EYFP-tagged viral expression (green), CRF mRNA (magenta), and SOM mRNA (red). Scale bar represents 10 µm. C, Comparison of the efficiency of our viral vector targeting strategy between CRF-Cre and SOM-Cre mice. D, Comparison of the specificity of our viral vector targeting strategy between CRF-Cre and SOM-Cre mice. E, Comparison of the cross-lineage leakage of our viral vector targeting strategy between CRF-Cre and SOM-Cre mice. CRF-Cre: n = 7, SOM-Cre: n = 8. FISH, fluorescence in situ hybridization. Data for C–E are presented as median ± interquartile range and were analyzed with Mann–Whitney test. **p < 0.01.